tlr2  (Cell Signaling Technology Inc)

Journal: The FASEB Journal

Article Title: SR‐717, a Non‐Nucleotide STING Agonist, Displayed Anti‐Radiation Activity in a IL‐6 Dependent Manner

doi: 10.1096/fj.202403127R

Figure Lengend Snippet: SR‐717 plays a role in intestinal radiation protection through TLR2 signaling pathway. (A) The KEGG pathway top 10 (Total) enrichment in the intestines of mice treated in the presence or absence of SR‐717. (B) GSEA of pattern recognition receptor activity. (C) GSEA of Tolls signaling pathway. (D) Heatmap of Toll‐like receptor signaling pathway genes. (E) RNA obtained from mice intestinal was used to perform the qRT‐PCR ( n = 3). (F) Western blot analysis of activation of the TLR2 signaling pathways in MODE‐K cells stimulated with SR‐717 ( n = 3). (G) TLR2 KO mice were treated with SR‐717 or saline before 9.5 Gy TBI. Pathological images and statistical analysis of the intestine in mice ( n = 3). (H) Organoid extracted from TLR2 KO mice, were then exposed to SR‐717 or PBS. And the relative area and budding condition of intestinal organoids. Data are represented as mean ± SEM. ns, non‐significance.

Article Snippet: Cells were treated as indicated and harvested for protein extraction, and then analyzed by western blotting to detect β‐Actin (Cell Signaling Technology, 4970S, 1:1000), STING (Cell Signaling Technology, 13647, 1:1000), p‐STING (Cell Signaling Technology, 72971, 1:1000), p‐TBK1 (Cell Signaling Technology, 5483, 1:1000), p‐p65 (Cell Signaling Technology, 8242T, 1:1000), STAT3 (Proteintech, 10253‐2‐AP, 1:2000), p‐STAT3 (Proteintech, 28945‐1‐AP, 1:1000), TLR2 (Cell Signaling Technology, 13744, 1:1000) and Myd88 (Cell Signaling Technology, 4283, 1:1000).

Techniques: Activity Assay, Quantitative RT-PCR, Western Blot, Activation Assay, Protein-Protein interactions, Saline